Media - YEM (YEast extract - Manitol)

by A. Untergasser (contact address and download at www.untergasser.de/lab)
Version: 1.0 - Print Version (.PDF)

This is a very basic recipe used to grow rhizobium strains.

Composition:

	1 liter	2 liter	4 liter	5 liter
Mannitol	5 g	10 g	20 g	25 g
Yeast extract	0.5 g	1 g	2 g	2.5 g
MgSO₄ x 7 H₂0	0.2 g	0.4 g	0.8 g	1 g
NaCl	0.1 g	0.2 g	0.4 g	0.5 g
K₂HPO₄	0.5 g	1 g	2 g	2.5 g
Na Gluconate	5 g	10 g	20 g	25 g
add water to	1 l	2 l	4 l	5 l

Prepare 16.6% CaCl₂ solution.

Autoclave for 20 min at 120 °C

Add 1 ml of the 16.6% CaCl₂ solution to 1 liter YEM medium (1:1000)

Let cool to ca. 55 °C before adding antibiotics

Store at room temperature or 4 °C

Plates:

Prepare YEM as described above, but add before autoclaving 1,5 % Agar (15 g per liter)

Materials needed:

Yeast Extract (# Y1333.1000) by Duchefa

References and Comments:

This is a recipe I ferequently used in Ton Bisselings Lab.

How to cite this page in publications:

This document can be cited like this:

Untergasser A. “Media - YEM (YEast extract - Manitol)” Untergasser's Lab. Summer 2008 (include here the date when you accessed these page).
<http://www.untergasser.de/lab/media/media_yem_v1_0.htm>.

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This article is copyrighted. Please do not reproduce this article in whole or part, in any form, without obtaining my written permission.